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HelpOrder Code PA Procainamide and N-Acetylprocainamide, Serum
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions:
1. Serum gel tubes should be centrifuged within 2 hours of collection.
2. Red-top tubes should be centrifuged and the serum aliquoted into a plastic vial within 2 hours of collection.
Useful For
Monitoring therapy with procainamide
Assessing compliance
Evaluating procainamide toxicity
Method Name
Homogeneous Enzyme Immunoassay
Reporting Name
Procainamide and NAPA, SSpecimen Type
SerumSpecimen Minimum Volume
0.25 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 14 days | |
| Refrigerated | 7 days | ||
| Ambient | 24 hours |
Reject Due To
| Gross hemolysis | Reject |
Reference Values
Procainamide
Therapeutic: 4.0-10.0 mcg/mL
Critical value: >12.0 mcg/mL
N-acetylprocainamide
Therapeutic: 12.0-18.0 mcg/mL
Critical value: ≥40.0 mcg/mL
Method Description
These assays are based on a homogeneous enzyme immunoassay technique. The assay is a competition assay between the drug in the sample and drug labeled with the enzyme glucose-6-phosphate dehydrogenase (G6PD) for antibody binding sites. Enzyme activity decreases upon binding to the antibody, so the drug concentration in the sample can be measured in terms of enzyme activity. Active enzyme converts oxidized nicotinamide adenine dinucleotide (NAD) to NADH, resulting in an absorbance change that is measured spectrophotometrically. Endogenous serum G6PD does not interfere because the coenzyme functions only with the bacterial (Leuconostoc mesenteroides) enzyme employed in the assay.(Package inserts: Procainamide and N-acetylprocainamide. Roche Diagnostics; 08/2015)
Day(s) Performed
Monday through Saturday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
80192
Forms
If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.