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HelpOrder Code PA Procainamide and N-Acetylprocainamide, Serum
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL Serum
Collection Instructions:
1. Within 2 hours of collection, centrifuge the specimen.
2. For serum gel tubes, plasma may sit on gel refrigerated or be aliquoted into a plastic vial.
3. For red top tubes, aliquot serum immediately into a plastic vial.
Useful For
Monitoring therapy with procainamide
Assessing compliance
Evaluating procainamide toxicity
Method Name
Homogeneous Enzyme Immunoassay
Reporting Name
Procainamide and NAPA, SSpecimen Type
SerumSpecimen Minimum Volume
Serum: 0.25 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Frozen (preferred) | 14 days |
| Refrigerated | 7 days | |
| Ambient | 24 hours |
Reject Due To
| Gross hemolysis | Reject |
Reference Values
Procainamide
Therapeutic: 4.0-10.0 mcg/mL
Critical value: >12.0 mcg/mL
N-acetylprocainamide
Therapeutic: 12.0-18.0 mcg/mL
Critical value: ≥40.0 mcg/mL
Method Description
These assays are based on a homogeneous enzyme immunoassay technique in which there is competition between drug in the sample and drug labeled with the enzyme glucose-6-phosphate dehydrogenase (G6PD) for antibody binding sites. Enzyme activity decreases upon binding to the antibody, so the drug concentration in the sample can be measured in terms of enzyme activity. Active enzyme converts oxidized nicotinamide adenine dinucleotide (NAD) to NADH, resulting in an absorbance change that is measured spectrophotometrically. Endogenous serum G6PD does not interfere because the coenzyme functions only with the bacterial (Leuconostoc mesenteroides) enzyme employed in the assay.(Package insert: PROC2. Roche Diagnostics; 02/2024; package insert: NAPA2. Roche Diagnostics; 12/2023)
Day(s) Performed
Monday through Saturday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
80192
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PA | Procainamide and NAPA, S | 3983-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8683 | Procainamide, S | 3982-6 |
| 2461 | N-acetylprocainamide, S | 3834-9 |
| 2462 | Procainamide + NAPA | 3983-4 |
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Cardiovascular Test Request (T724)
-Therapeutics Test Request (T831)