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Order Code PCHE1 Pseudocholinesterase, Total, Serum


Necessary Information


Patient's age and sex are required.



Specimen Required


Patient Preparation: For patients with prolonged apnea following surgery, wait at least 24 hours before obtaining specimen.

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Serum gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged, and the serum aliquoted into a plastic vial within 2 hours of collection.


Useful For

Monitoring exposure to organophosphorus insecticides and herbicides

 

Monitoring patients with liver disease, particularly those undergoing liver transplantation

 

Identifying patients who are homozygous or heterozygous for an atypical gene and have low levels of pseudocholinesterase

 

This test is not useful for the differential diagnosis of jaundice.

Method Name

Colorimetric Assay

Reporting Name

Pseudocholinesterase, Total, S

Specimen Type

Serum

Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  365 days
  Ambient  24 hours

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Reference Values

Males

5320-12,920 U/L

 

Females

0-15 years: 5320-12,920 U/L

16-39 years: 4260-11,250 U/L

40-41 years: 5320-12,920 U/L

≥42 years: 5320-12,920 U/L

Note: Females aged 18-41 years who are pregnant or taking hormonal contraceptives, the reference interval is 3650-9120 U/L.

Method Description

Cholinesterase catalyzes the hydrolysis of butyrylthiocholine to thiocholine and butyrate. Thiocholine instantaneously reduces the yellow hexacyanoferrate (III) to the almost colorless hexacyanoferrate (II). This decrease in color can be measured photometrically.(Package insert: Cholinesterase Gen 2 Reagent. Roche Diagnostics; V.10.0, 01/2022)

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

82480