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HelpOrder Code PEANT Peanut, IgE with Reflex to Peanut Components, IgE, Serum
Useful For
Evaluating patients with suspected peanut allergy
Evaluating patients with possible peanut cross-reactivity
This test is not useful for patients previously treated with immunotherapy to determine if residual clinical sensitivity exists or in whom the medical management does not depend upon identification of allergen specificity.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| PNTCP | Peanut Components, IgE, S | No | No |
Testing Algorithm
Testing begins with analysis of peanut IgE. If peanut IgE is negative (<0.10 kU/L), testing is complete.
If peanut IgE is 0.10 kU/L or greater, then 7 peanut components (Ara h 2, Ara h 1, Ara h 3, Ara h 6, Ara h 8, Ara h 9, and profilin Bet v2) are performed at an additional charge.
Method Name
Fluorescent Enzyme Immunoassay (FEIA)
Reporting Name
Peanut Component Reflex, SSpecimen Type
SerumOrdering Guidance
For a listing of allergens available for testing, see Allergens - Immunoglobulin E (IgE) Antibodies.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 2 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 14 days |
| Frozen | 90 days | |
| Ambient | 7 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
|
Class |
IgE kU/L |
Interpretation |
|
0 |
<0.10 |
Negative |
|
0/1 |
0.10-0.34 |
Borderline / Equivocal |
|
1 |
0.35-0.69 |
Equivocal |
|
2 |
0.70-3.49 |
Positive |
|
3 |
3.50-17.4 |
Positive |
|
4 |
17.5-49.9 |
Strongly positive |
|
5 |
50.0-99.9 |
Strongly positive |
|
6 |
≥100 |
Strongly positive |
Concentrations of 0.70 kU/L or more (class 2 and above) will flag as abnormally high.
Reference values apply to all ages.
Method Description
Specific IgE from the patient's serum reacts with the allergen of interest, which is covalently coupled to an ImmunoCAP. After washing away nonspecific IgE, enzyme-labeled anti-IgE antibody is added to form a complex. After incubation, unbound anti-IgE is washed away, and the bound complex incubated with a developing agent. After stopping the reaction, the fluorescence of the eluate is measured. Fluorescence is proportional to the amount of specific IgE present in the patient's sample (ie, the higher the fluorescence value, the more IgE antibody is present).(Package insert: ImmunoCAP System Specific IgE FEIA. Phadia; Rev 06/2020)
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86003
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PEANT | Peanut Component Reflex, S | 6206-7 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| PNUT | Peanut, IgE, S | 6206-7 |
Forms
If not ordering electronically, complete, print, and send an Allergen Test Request (T236) with the specimen.