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HelpOrder Code PERA Preeclampsia sFlt-1/PIGF (Soluble fms-Like Tyrosine Kinase 1/ Placental Growth Factor) Ratio, Serum
Ordering Guidance
The test is indicated for use in pregnant women, with singleton pregnancies (gestational age 23 to 34+6/7 weeks) hospitalized for hypertensive disorders of pregnancy (preeclampsia, chronic hypertension with or without superimposed preeclampsia or gestational hypertension), within 2 weeks of presentation.
Specimen Required
Patient Preparation: For 24 hours before specimen collection, the patient should not receive intravenous heparin.
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Aiding in risk assessment of patients with clinical signs and symptoms consistent with development of preeclampsia with severe features
This test is not intended for making a diagnosis of preeclampsia or preeclampsia with severe features.
This test is not a stand-alone test for monitoring of hypertensive disorders of pregnancy or for changing treatment, including medication.
Method Name
Immunofluorescent Assay (IFA)
Reporting Name
Preeclampsia sFlt-1/PlGF Ratio, SSpecimen Type
SerumSpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 180 days | |
| Refrigerated | 24 hours |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | Reject |
Reference Values
<40
Method Description
The B·R·A·H·M·S sFlt-1 (soluble fms-like tyrosine kinase 1) KRYPTOR and B·R·A·H·M·S PlGF (placental growth factor) plus KRYPTOR are homogeneous sandwich immunoassays for detection of sFlt-1 or PIGF, respectively, in human serum. The measuring principle is based on time-resolved amplified cryptate emission (TRACETM) technology, which measures the signal that is emitted from an immunocomplex with time delay. When the sample is excited with a nitrogen laser at 337 nm, the donor (eg, cryptate) emits a long-life fluorescent signal in the millisecond range (eg, at 620 nm), while the acceptor (eg, XL) generates a short-life signal in the nanosecond-range (eg, at 707 nm). When the two components are bound in an immunocomplex, both the signal amplification and the prolongation of the life span of the acceptor signal occur at 707 nm, so that it can be measured over microseconds. Signal detection is delayed by 50 microseconds to isolate the long signal emitted by immunocomplex from short signals of unbound fluorophores. This long-lived signal, which is proportional to the concentration of the analyte of interest is measured by selecting the spectral and temporal information.(Package inserts: BRAHMS sFlt-1 Kryptor. Thermo Fisher Scientific BRAHMS LLC; Version 1.Ous, 05/2023; BRAHMS PlGF PLUS Kryptor. Thermo Fisher Scientific BRAHMS LLC; Version R2.Ous, 09/2023)
Day(s) Performed
Monday through Saturday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
0482U