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HelpOrder Code PF199 Carbohydrate Antigen 19-9 (CA 19-9), Pleural Fluid
Reporting Name
CA 19-9, Pleural FluidUseful For
An adjuvant to cytology and imaging studies to differentiate between nonmalignant and malignant causes of pleural effusions
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Pleural FluidSpecimen Required
Patient Preparation: For 12 hours before specimen collection, patient should not take multivitamins or dietary supplements (eg, hair, skin, and nail supplements) containing biotin (vitamin B7).
Source: Pleural fluid, thoracentesis fluid
Container/Tube: Plain, plastic, screw-top tube
Specimen Volume: 2 mL
Specimen Minimum Volume
0.5 mL (Samples <0.5 mL may be rejected)
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Pleural Fluid | Frozen (preferred) | 90 days |
| Refrigerated | 14 days | |
| Ambient | 7 days |
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Saturday
CPT Code Information
86301
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PF199 | CA 19-9, Pleural Fluid | 19163-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| P199 | CA 19-9, Pleural Fluid | 19163-5 |
| SITE8 | Site | 39111-0 |
Method Description
The instrument used is a Beckman Coulter DXI 800. The Access GI Monitor assay is a 2-site immunoenzymatic sandwich assay. A sample is added to a reaction vessel along with paramagnetic particles coated with polyclonal goat antibiotin antibody, mouse monoclonal biotin conjugate, and buffered protein solution. After incubation in a reaction vessel, separation in a magnetic field, and washing to remove materials not bound to the solid phase, a monoclonal-alkaline phosphatase conjugate is added. After incubation in a reaction vessel, materials bound to the solid phase are held in a magnetic field, while unbound materials are washed away. The chemiluminescent substrate Lumi-Phos 530 is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is directly proportional to the concentration of carbohydrate antigen 19-9 antigen in the sample. The amount of analyte in the sample is determined from a stored, multipoint calibration curve.(Package insert: Access GI Monitor assay. Beckman Coulter, Inc.; 2020)
Reject Due To
| Gross hemolysis | Reject |
| Gross icterus | OK |
Method Name
Immunoenzymatic Assay
Forms
If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.