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Order Code PLABN Platelet Antibody Screen, Serum

Useful For

Detecting alloantibodies to epitopes on platelet glycoproteins IIb/IIIa, Ib/Ix, Ia/IIa, IV and class I human leukocyte antigens to evaluate cases of immune mediated refractoriness to platelet transfusions, posttransfusion purpura, or neonatal alloimmune thrombocytopenia

Method Name

Solid Phase Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

Platelet Ab Screen, S

Specimen Type

Serum Red


Ordering Guidance


For testing neonates, send a maternal specimen, as unbound platelet antibodies may not be detected in the neonate's serum.

 

This test is not recommended for the diagnosis of immune thrombocytopenia or autoimmune thrombocytopenia. Tests that are optimized to detect antibodies bound to the platelets will be useful in these situations; cell-bound platelet antibody (direct) test is strongly recommended.



Necessary Information


 



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL Serum

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.

Additional Information: For testing neonates, draw blood from the mother of the neonate. Then follow the Collection Instructions prior to sending.


Specimen Minimum Volume

Serum: 0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Frozen (preferred) 365 days
  Refrigerated  48 hours

Reject Due To

Gross hemolysis Reject

Reference Values

Not applicable

Method Description

Patient serum is added to microwells coated with platelet and human leukocyte antigen (HLA) glycoproteins, allowing antibody, if present, to bind. Unbound antibodies are then washed away. An alkaline phosphatase-labeled antihuman globulin reagent (anti-IgG/A/M) is added to the microwells and incubated. The unbound anti-IgG/A/M is washed away and the substrate p-nitrophenylphosphate (PNPP) is added to the wells and incubated. The reaction is stopped with stopping solution. The optical density of the color that develops is measured in a spectrophotometer and results are interpreted.(Package insert: PakPlus. Immucor GTI Diagnostics; 303469.IFUEN Rev E; 07/2015)

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86022

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PLABN Platelet Ab Screen, S 95270-5

 

Result ID Test Result Name Result LOINC Value
PTL01 Overall Result 24375-8
PTL02 Interpretation 59466-3
PTL03 GPIIb/IIIa (Cell-1) 48505-2
PTL04 GPIIb/IIIa (Cell-2) 48505-2
PTL05 GPIa/IIa (Cell-1) 47084-9
PTL06 GPIa/IIa (Cell-2) 47084-9
PTL07 GPIb/IX 48506-0
PTL08 GPIV 87757-1
PTL09 HLA Class I 95269-7

Testing Algorithm

For more information see Platelet Antibody Testing Algorithm.