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HelpOrder Code PLAFL Platelet Surface Glycoprotein by Flow Cytometry, Blood
Useful For
Identification of markedly decreased CD41 (GPIIb) and CD61 (GPIIIa) expression levels, which are diagnostic for Glanzmann thrombasthenia
Identification of markedly decreased CD42a (GPIX) and CD42b (GPIb-alpha) expression levels, which are diagnostic for Bernard-Soulier syndrome
Identification of decreased GPVI expression, which suggests collagen receptor deficiency
Identification of decreased CD49b (GPIa), which suggests collagen receptor deficiency
Special Instructions
Method Name
Immunophenotyping
Reporting Name
Platelet Glycoprotein Flow, BSpecimen Type
Whole Blood ACDShipping Instructions
Specimen must be shipped ambient and arrive within 4 days of draw.
Ship specimen overnight in an Ambient Shipping Box-Critical Specimens Only (T668) following the instructions in the mailer.
Necessary Information
Platelet Esoteric Testing Patient Information is required. Testing may proceed without the patient information, however, the information aids in providing a more thorough interpretation. Ordering providers are strongly encouraged to fill out the form and send with the specimen.
Specimen Required
Supplies: Ambient Shipping Box-Critical Specimens Only (T668)
Collection Container/Tube: ACD solution A or B
Specimen Volume: 6 mL
Pediatric Volume: 1 mL
Collection Instructions: Do not transfer blood to other containers.
Specimen Minimum Volume
Adult: 1 mL
Pediatric 200 mcL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood ACD | Ambient | 4 days |
Reject Due To
| Gross hemolysis | Reject |
| Fully Clotted | Reject |
| Gross lipemia | OK |
Reference Values
GPIIb CD41: ≥70.0% (Normal Range-Median)
GPIIIa CD61: ≥70.0% (Normal Range-Median)
GPIX CD42a: ≥70.0% (Normal Range-Median)
GPIb-alpha CD42b: ≥70.0% (Normal Range-Median)
GPIa CD49b: ≥60.0% (Normal Range-Median)
Method Description
Flow cytometric immunophenotyping of peripheral blood platelets is performed using the following antibodies:
Panel: CD41 (IIb), CD42a (IX), CD42b (Ib-alpha), CD49b (GPIa), CD61 (GPIIIa), and GPVI.
For sample quality purposes, CD62P is evaluated.
Using whole blood collected in ACD (A or B), platelet surface GPIa, Ib-alpha, IIb, IIIa, VI and IX expression levels are measured by flow cytometry method. Platelets in whole blood are stained with various fluorochrome-labeled primary antibodies and fixed. Then the platelet surface fluorescent intensities of various bound antibodies are measured by flow cytometers. Platelets are first gated by forward and side scatter. Mean fluorescent intensities are recorded and converted to percentage of a median fluorescent intensity of a normal donor study of 20 healthy donors. If the percentage of expression of a glycoprotein (GP) is lower than the corresponding normal range, a deficiency of a GP is detected.(Unpublished Mayo Method)
Day(s) Performed
Monday through Saturday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
88184-Flow cytometry; first cell surface, cytoplasmic or nuclear marker
88185-Flow cytometry; additional cell surface, cytoplasmic or nuclear marker (each) X5
88187-Flow cytometry interpretation, 2 to 8 markers
Forms
1. Platelet Esoteric Testing Patient Information is required.
2. If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.