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Order Code PLPMA Prolactin, Pituitary Macroadenoma, Serum

Useful For

Quantifying prolactin in serum specimens where the high-dose hook effect is suspected (eg, presence of pituitary tumor with symptoms of prolactinoma, and lower than expected serum prolactin concentration)

Testing Algorithm

A pituitary adenoma should be identified by imaging studies prior to ordering this test.

Reporting Name

Prolactin, Pituitary Macroadenoma

Specimen Type

Serum


Ordering Guidance


For initial patient assessment; order PRL / Prolactin, Serum as the screening test.



Necessary Information


Patient's age and sex are required.



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 2 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

1.75 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
  Frozen  90 days
  Ambient  24 hours

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Reference Values

Males

<18 years: not established

≥18 years: 4.0-15.2 ng/mL

 

Females:

<18 years: not established

≥18 years: 4.8-23.3 ng/mL 

Method Description

The Roche Cobas e immunoassay Prolactin II method employs 2 monoclonal antibodies specifically directed against prolactin. A biotinylated monoclonal antibody and a second monoclonal antibody labeled with a ruthenium complex react with prolactin in the sample to form a sandwich complex. After the addition of streptavidin-coated microparticles, the complex becomes bound to the solid phase via interaction of biotin and streptavidin. Application of a voltage to the electrode then induces chemiluminescent emission, which is measured.(Package insert: Roche E170/Cobas e601/e602 Prolactin II)

 

Dilution of serum sample is performed (10-, 100-, and 400-fold dilution) using manufacturer-recommended diluent and recovery of prolactin is compared to the prolactin concentration in neat sample. Acceptable recovery is defined as 100 + or - 20% recovery after dilution of serum.

Day(s) Performed

Monday through Sunday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

84146

Method Name

Electrochemiluminescent Immunoassay

Forms

If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.