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Order Code QFEVR Q Fever Antibody Screen with Titer Reflex, Serum


Ordering Guidance


 



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.6 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Screening for exposure to Coxiella burnetii, the causative agent of Q fever

 

This test should not be used as a screening procedure for the general population.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
QFP Q Fever IgM/IgG, Titer, S No No

Testing Algorithm

If the Q fever serology result is reactive, then Q fever antibody confirmation by indirect immunofluorescence will be performed at an additional charge.

 

For more information see Infective Endocarditis: Diagnostic Testing for Identification of Microbiological Etiology.

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

Q Fever Ab Scrn w/ Titer Reflex, S

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Heat-inactivated specimen Reject

Reference Values

Negative

Reference values apply to all ages

Method Description

The test uses microplate strips, each with 8 break-off reagent wells coated with purified native antigens from Coxiella burnetii cells in the acute and chronic phase. In the first reaction step, diluted samples are incubated in the wells. In the case of positive samples, specific IgA, IgG, or IgM antibodies will bind to the antigens. To detect the bound antibodies, a second incubation is carried out using an enzyme-labelled anti-human IgA-G-M (enzyme conjugate) catalyzing a color reaction.(Unpublished Mayo method)

Day(s) Performed

Sunday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86638

86638 x 4 (if applicable)

Forms

If not ordering electronically, complete, print, and send an Infectious Disease Serology Test Request (T916) with the specimen.