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Order Code QFP Q Fever IgM and IgG, Titer, Serum

Reporting Name

Q Fever IgM/IgG, Titer, S

Useful For

Diagnosis of Coxiella burnetii, the causative agent of Q fever

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Ordering Guidance


 



Specimen Required


Only orderable as a reflex. For more information see QFEVR / Q Fever Antibody Screen with Titer Reflex, Serum.

 

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Only orderable as a reflex. For more information see QFEVR / Q Fever Antibody Screen with Titer Reflex, Serum.

 

Q fever phase I antibody, IgG

<1:16

 

Q fever phase II antibody, IgG

<1:16

 

Q fever phase I antibody, IgM

<1:16

 

Q fever phase II antibody, IgM

<1:16

 

Reference values apply to all ages.

Day(s) Performed

Monday through Saturday

CPT Code Information

86638 x 4

Method Description

An indirect immunofluorescence test is used for the measurement of IgM and IgG antibodies to Coxiella burnetii. Specific antibodies present in the serum of the patient react with rickettsial cells that have been previously fixed on a glass microscope slide. Fluorescein-labeled antihuman IgG or IgM conjugate is used to stain specific antibody bound to the substrate cells. The slides are examined with a fluorescence microscope for characteristic, apple-green fluorescence of the infected cell.(Edligner B. Immunofluorescence serology. A tool for prognosis of Q fever. Diagn Microbiol Infect Dis. 1985;3[4]:343-351; package inserts: Q fever IFA IgG. Focus Diagnostics, Inc; 12/2022; Q fever IFA IgM. Focus Diagnostics, Inc; 12/2022)

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Method Name

Only orderable as a reflex. For more information see QFEVR / Q Fever Antibody Screen with Titer Reflex, Serum.

 

Indirect Immunofluorescence