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HelpOrder Code RO52 Ro52 Antibody, IgG, Serum
Ordering Guidance
This test detects antibodies to Ro52 only. For testing antibodies to both Ro 52 and Ro60, order ROPAN/Ro52 and Ro60 Antibodies, IgG, Serum.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial
Useful For
Evaluating patients at risk for connective tissue disease with or without interstitial lung disease
Differentiating single vs double positivity for Ro52 and Ro60 antibodies in combination with anti-Ro60
Method Name
Chemiluminescent Immunoassay
Reporting Name
Ro52 Antibody, IgG, SSpecimen Type
SerumSpecimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 14 days |
| Frozen | 21 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
Reference Values
<20 CU (negative)
≥20 CU (positive)
Reference values apply to all ages
Method Description
Paramagnetic beads are coated with purified recombinant Ro52 protein. The serum sample is diluted in assay buffer and incubated with the beads. Antibodies to Ro52 bind to their respective beads and are detected by anti-human IgG antibody conjugated with isoluminol. With addition of trigger reagents, a luminescent reaction is produced by the isoluminol conjugate. The light produced by the reaction is measured by a photomultiplier, proportional to the amount of antibodies that are bound to the beads. The antibodies are quantified according to the working curve on the instrument. Anti-Ro52 are reported in chemiluminescent units as derived from the relative light units measured from each sample.(Package insert: QUANTA Flash Ro52 Reagents, Inova Diagnostics; 06/2019)
Day(s) Performed
Wednesday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86235