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HelpOrder Code ROPAN Ro52 and Ro60 Antibodies, IgG, Serum
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial
Useful For
Evaluating patients at risk for connective tissue disease with or without interstitial lung disease
Differentiation of antibodies to Ro52 and Ro60 in patients known to be positive for anti-SS-A (Ro) antibodies
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| RO52 | Ro52 Antibody, IgG, S | Yes | Yes |
| RO60 | Ro60 Antibody, IgG, S | Yes | Yes |
Method Name
Chemiluminescent Immunoassay
Reporting Name
Ro52 and Ro60 Antibodies, IgG, SSpecimen Type
SerumSpecimen Minimum Volume
0.8 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 21 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
Reference Values
Ro52 ANTIBODY, IgG
<20 CU (negative)
≥20 CU (positive)
Reference values apply to all ages
Ro60 ANTIBODY, IgG
<20 CU (negative)
≥20 CU (positive)
Reference values apply to all ages
Method Description
Paramagnetic beads are coated with purified recombinant Ro52 protein or purified recombinant Ro60 protein. The serum sample is diluted in assay buffer and incubated with the beads. Antibodies to Ro52 or Ro60 bind to their respective beads and are detected by anti-human IgG antibody conjugated with isoluminol. With addition of trigger reagents, a luminescent reaction is produced by the isoluminol conjugate. The light produced by the reaction is measured by a photomultiplier, proportional to the amount of antibodies that are bound to the beads. The antibodies are quantified according to the working curve on the instrument. Anti-Ro52 and anti-Ro60 are reported in Chemiluminescent Units (CU) as derived from the relative light units measured from each sample.(Package insert: QUANTA Flash Ro52 Reagents, Inova Diagnostics; 06/2019; QUANTA Flash Ro60 Reagents, Inova Diagnostics; 10/2019)
Day(s) Performed
Wednesday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86235 x 2