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Order Code SALCT Cortisol, Saliva

Reporting Name

Cortisol, Saliva

Useful For

Screening for Cushing syndrome

 

Diagnosis of Cushing syndrome in patients presenting with symptoms or signs suggestive of the disease

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Saliva


Necessary Information


Collection time is required.



Specimen Required


Patient Preparation:

1. Do not brush teeth before collecting specimen.

2. Do not eat or drink for 15 minutes prior to specimen collection.

Supplies: Cortisol, Saliva Collection Kit (T514)

Container/Tube: SARSTEDT Salivette

Specimen Volume: 1.5 mL

Collection Instructions:

1. Provide patient with a Saliva Collection Kit (Salivette) containing the Cortisol - Saliva Collection Instructions and ask them to follow the instructions as written.

2. Instruct patient to collect specimen between 11 p.m. and midnight and record collection time on the Cortisol - Saliva Collection Instructions sheet.

3. Instruct patient to return Cortisol - Saliva Collection Instructions with the appropriately labeled Salivette to the laboratory.

Additional Information:

1. Reference values are also available for an 8 a.m. (7 a.m.-9 a.m.) or a 4 p.m. (3 p.m.-5 p.m.) collection, however, the 11 p.m. to midnight collection is preferred.

2. If multiple specimens are collected, submit each vial under a separate order.


Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Saliva Refrigerated (preferred) 28 days
  Frozen  60 days
  Ambient  28 days

Reference Values

7 a.m.-9 a.m.: 100-750 ng/dL

3 p.m.-5 p.m.: <401 ng/dL

11 p.m.-midnight: <100 ng/dL

Day(s) Performed

Monday through Friday

CPT Code Information

82533

Method Description

Isotopically labeled cortisol and cortisone are added to the sample as internal standards. Cortisol, cortisone, and coinciding internal standards are extracted from the specimens using online turbulent flow chromatography, which is followed by liquid chromatography tandem mass spectrometry analysis. Although cortisone is not reported, it is used to verify the cortisol value and screen for possible contamination from exogenous sources.(Unpublished Mayo method)

Reject Due To

  All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Forms

If not ordering electronically, complete, print, and send General Request (T239)