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HelpOrder Code SASP Aspergillus fumigatus, IgG Antibodies, Serum
Reporting Name
Aspergillus fumigatus, IgG Ab, SUseful For
Evaluation of patients suspected of having hypersensitivity pneumonitis (HP) induced by exposure to Aspergillus fumigatus
Evaluation of patients suspected of having HP who have documented environmental exposures to high-humidity environments
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 21 days | |
| Frozen | 21 days |
Reference Values
<4 years: not established
≥4 years: ≤102 mg/L
CPT Code Information
86606
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | Reject |
Method Name
Fluorescence Enzyme Immunoassay (FEIA)
Day(s) Performed
Monday through Friday
Method Description
The Phadia ImmunoCAP System specific IgG fluorescent enzyme immunoassay provides an in vitro method for measuring the concentrations of circulating specific IgG antibodies in human blood samples. Specific IgG from the patient's serum reacts with the antigen of interest, which is covalently coupled to an ImmunoCAP. After washing away nonspecific IgG, enzyme-labeled anti-IgG antibodies are added to form a complex. After incubation, unbound enzyme-anti IgG is washed away, and the bound complex is then incubated with a developing agent. After stopping the reaction, the fluorescence of the eluate is measured. The fluorescence is proportional to the amount of specific IgG, which is present in the patient's sample, ie, the higher the fluorescence value, the more specific IgG antibody is present.(Package insert: ImmunoCAP Aspergillus. Phadia AB; 10/2019)