Home
HelpOrder Code SECOS Secobarbital, Serum
Reporting Name
Secobarbital, SUseful For
Monitoring secobarbital therapy
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Serum RedSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Red top (Serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL
Collection Instructions:
1. Collect specimen immediately before the next scheduled dose.
2. Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.6 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum Red | Refrigerated (preferred) | 28 days |
| Ambient | 28 days | |
| Frozen | 28 days |
Reference Values
Therapeutic concentration: 1.0-2.0 mcg/mL
Toxic concentration: >5.0 mcg/mL
Cutoff concentrations by gas chromatography mass spectrometry:
Secobarbital: 0.5 ng/mL
Day(s) Performed
Thursday
CPT Code Information
80299
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| SECOS | Secobarbital, S | 4028-7 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8243 | Secobarbital, S | 4028-7 |
Method Description
Barbiturates are extracted from serum using solid-phase extraction techniques. The serum is buffered and eluted with organic solvent. The organic phase is dried, reconstituted, and analysis performed by gas chromatography-mass spectrometry using selected ion monitoring. The assay utilizes deuterated barbiturates as internal standards.(Unpublished Mayo method)
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Method Name
Gas Chromatography Mass Spectrometry (GC-MS)
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Neurology Specialty Testing Client Test Request (T732)
-Therapeutics Test Request (T831)