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Order Code SIIRO Sirolimus, Whole Blood

Reporting Name

Sirolimus, B

Useful For

Monitoring whole blood sirolimus concentration during therapy, particularly in individuals coadministered cytochrome P450 (CYP) 3A4 substrates, inhibitors, or inducers

 

Adjusting dose to optimize immunosuppression while minimizing toxicity

 

Evaluating patient compliance

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Whole Blood EDTA


Specimen Required


Container/Tube: Lavender top (EDTA)

Specimen Volume: 3 mL

Collection Instructions:

1. Draw blood immediately before a scheduled dose.

2. Do not centrifuge.

3. Send whole blood specimen in original tube. Do not aliquot.

Additional Information: Therapeutic range applies to trough specimen collected immediately prior to a.m. dose.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Refrigerated (preferred) 28 days
  Ambient  28 days
  Frozen  28 days

Reference Values

4-20 ng/mL (Trough)

 

Target steady-state trough concentrations vary depending on the type of transplant, concomitant immunosuppression, clinical/institutional protocols, and time post-transplant. Results should be interpreted in conjunction with this clinical information and any physical signs/symptoms of rejection/toxicity.

Day(s) Performed

Monday through Sunday

CPT Code Information

80195

Method Description

Blood specimens are subjected to protein precipitation. The resulting supernatant is analyzed by liquid chromatography tandem mass spectrometry.(Pablo AH, Breaud AR, Clarke W. Analysis of immunosuppressant drugs in whole blood by liquid chromatography-tandem mass spectrometry [LC-MS/MS]. Curr Protoc Toxicol. 2020;84(1):e92. doi:10.1002/cptx.92)

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK
Clotted specimens Reject

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Renal Diagnostics Test Request (T830)

-Therapeutics Test Request (T831)