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Order Code SLA Soluble Liver Antigen (SLA) IgG Antibodies, Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL Serum

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Forms

If not ordering electronically, complete, print, and send Gastroenterology and Hepatology Test Request (T728) with the specimen.

Useful For

Evaluation of patients at-risk for autoimmune hepatitis or liver disease of unknown etiology

Testing Algorithm

For information see First-Line Screening for Autoimmune Liver Disease Algorithm.

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

Soluble Liver Antigen Ab, IgG, S

Specimen Type

Serum

Specimen Minimum Volume

Serum: 0.4 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK
Heat treated specimens Reject

Reference Values

Negative: <20.0 U

Equivocal: 20.0-24.9 U

Positive: ≥ 25.0 U

Method Description

Partially purified, full-length recombinant human soluble liver antigen (SLA) is bound to the wells of a polystyrene microwell plate under conditions that will preserve the antigen in its native state. Prediluted controls and diluted patient sera are added to separate wells, allowing any SLA antibodies present to bind to the immobilized antigen. Unbound sample is washed away and an enzyme labeled anti-human IgG conjugate is added to each well. A second incubation allows the enzyme labeled anti-human IgG to bind to any patient antibodies that have become attached to the microwells. After washing away any unbound enzyme labeled anti-human IgG, the remaining enzyme activity is measured by adding a chromogenic substrate and measuring the intensity of the color that develops. The assay can be evaluated spectrophotometrically by measuring and comparing the color intensity that develops in the patient wells with the color in the control wells.(Package insert: QUANTA Lite SLA ELISA 708775. INOVA Diagnostics; Rev. 6, 11/2021)

Day(s) Performed

Monday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

83516

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SLA Soluble Liver Antigen Ab, IgG, S 32219-8

 

Result ID Test Result Name Result LOINC Value
SLA Soluble Liver Antigen Ab, IgG, S 32219-8