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Order Code SSA SS-A/Ro Antibodies, IgG, Serum

Additional Codes

Mayo Test ID
SSA

Reporting Name

SS-A/Ro Ab, IgG, S

Useful For

Evaluating patients at-risk for connective tissue disease with or without interstitial lung disease

 

Detection of both anti-SS-A 52 (Ro52) and SS-A 60 (Ro60) antibodies in serum

Specimen Type

Serum


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Information: Centrifuge and aliquot serum into plastic vial.


Specimen Minimum Volume

0.35 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK
Heat-treated specimen Reject

Reference Values

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Day(s) Performed

Monday through Saturday

Method Name

Multiplex Flow Immunoassay

CPT Code Information

86235

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SSA SS-A/Ro Ab, IgG, S 33610-7

 

Result ID Test Result Name Result LOINC Value
SSA SS-A/Ro Ab, IgG, S 33610-7

Testing Algorithm

For more information see Connective Tissue Disease Cascade.

Special Instructions

Method Description

Recombinant SS-A/Ro 52 kD and affinity-purified SS-A/Ro 60 kD antigens are coupled covalently to polystyrene microspheres, which are impregnated with fluorescent dyes to create a unique fluorescent signature. SS-A/Ro antibodies, if present in diluted serum, bind to the SS-A/Ro antigens on the microspheres. The microspheres are washed to remove extraneous serum proteins. Phycoerythrin (PE)-conjugated antihuman IgG antibody is then added to detect IgG anti-SS-A/Ro bound to the microspheres. The microspheres are washed to remove unbound conjugate, and bound conjugate is detected by laser photometry. A primary laser reveals the fluorescent signature of each microsphere to distinguish it from microspheres that are labeled with other antigens, and a secondary laser reveals the level of PE fluorescence associated with each microsphere. Results are calculated by comparing the median fluorescence response for SS-A/Ro microspheres to a 4-point calibration curve.(Package insert: Bioplex 2200 ANA Screen. Bio-Rad Laboratories, 02/2019)