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Order Code SUBIF Immunoglobulin G (IgG) Subtypes Immunofluorescence, Tissue


Ordering Guidance


If additional interpretation/analysis is needed, request PATHC / Pathology Consultation along with this test and send the corresponding renal pathology light microscopy and immunofluorescence (IF) slides (or IF images on a CD), electron microscopy images (prints or CD), and the pathology report.



Shipping Instructions


1. Advise shipping specimens in Styrofoam transportation coolers to avoid extreme hot or cold temperatures to ensure specimens are received at required specimen stability temperature.

2. Attach the green pathology address label included in the kit to the outside of the transport container.



Necessary Information


A pathology/diagnostic report is required.



Specimen Required


Preferred: Frozen tissue

Supplies: Renal Biopsy Kit (T231)

Specimen Type: Kidney tissue

Container/Tube: Renal Biopsy Kit, Zeus/Michel's, Frozen

Specimen Volume: Entire specimen

Collection Instructions: Collect specimens according to the instructions in Renal Biopsy Procedure for Handling Tissue for Light Microscopy, Immunofluorescent Histology, and Electron Microscopy.

Additional Information: If standard immunoglobulin and complement immunofluorescence has already been performed, submit the residual frozen tissue (must contain glomeruli) on dry ice.

 

Acceptable: Frozen tissue

Slides: 4 frozen tissue unstained positively-charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.


Forms

1. Renal Biopsy Patient Information

2. If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.

Useful For

Determining the subclass of IgG antibody found in renal immunofluorescent panel and determining if the deposits are monoclonal or monotypic

Method Name

Direct Immunofluorescence

Reporting Name

IGG Subtypes Immunofluorescence

Specimen Type

Special

Specimen Stability Information

Specimen Type Temperature Time Special Container
Special Frozen (preferred)
  Ambient 
  Refrigerated 

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Method Description

Direct immunofluorescence staining on sections of fresh or frozen tissue.(Unpublished Mayo method)

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

88346-primary IF

88350-if additional IF