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HelpOrder Code TB1LN Antimicrobial Susceptibility, Mycobacterium tuberculosis Complex, First Line, Varies
Infectious
Ordering Guidance
If testing for the first-line agent pyrazinamide is desired, order MTBPZ/Mycobacterium tuberculosis Complex, Pyrazinamide Resistance by pncA DNA Sequencing, Varies.
Additional Testing Requirements
If organism identification is not provided, CTB / Mycobacteria and Nocardia Culture, Varies or CTBID / Culture Referred for Identification, Mycobacterium and Nocardia, Varies must also be ordered and will be charged separately.
Shipping Instructions
1. See Infectious Specimen Shipping Guidelines in Special Instructions.
2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.
Necessary Information
Specimen source and suspected organism identification are required.
Specimen Required
Specimen Type: Organism
Supplies: Infectious Container, Large (T146)
Container/Tube: Middlebrook 7H10 agar slant
Specimen Volume: Isolate
Collection Instructions: Organism must be in pure culture, actively growing.
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
Useful For
Susceptibility testing of Mycobacterium tuberculosis complex isolates growing in pure culture.
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| STV1 | Susceptibility, Mtb Complex, Broth | No, (Bill Only) | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| TB2LN | Susceptibility, Mtb Complex, 2 Line | Yes | No |
Testing Algorithm
When this test is ordered, the additional test will always be performed and charged separately.
If resistance to a first line antimicrobial agent is detected, reflex testing of second line agents will be performed and charged.
Special Instructions
Method Name
Broth Dilution at Critical Drug Concentrations
Reporting Name
Susceptibility, Mtb Complex, 1 LineSpecimen Type
VariesSpecimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Reject Due To
| Agar plate | Reject |
Reference Values
Results are reported as susceptible or resistant.
Method Description
This test method is based on growth or absence of growth of Mycobacterium tuberculosis complex isolates in broth cultures containing critical concentrations of the antimycobacterial agents isoniazid, rifampin, and ethambutol. The FDA-cleared platform BACTEC MGIT 960 (Becton Dickinson) is used.(Brown-Elliott, BA, Cirillo DM, Musser KA, Rowlinson M-C. Susceptibility Test Methods: Mycobacteria, Nocardia, and Other Actinomycetes. In: Carroll KC, Pfaller MA, eds. Manual of Clinical Microbiology, 13th Edition. ASM Press, 2023)
The BACTEC MGIT 960 platform measures fluorescence in a Mycobacterial Growth Indicator Tube (MGIT) containing an M tuberculosis complex isolate in the presence of critical concentrations of an antimycobacterial agent. Antimycobacterial agents tested are isoniazid, rifampin, and ethambutol. (Package insert: BACTEC MGIT 960 SIRE Kit, BD Diagnostics, 2016)
Day(s) Performed
Monday through Sunday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87188 x 3-Antimicrobial Susceptibility, Mycobacterium tuberculosis Complex, Broth Method