Sign in →

Order Code THEO Theophylline, Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Serum gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and the serum aliquoted into a plastic vial within 2 hours of collection.


Useful For

Assessing and adjusting theophylline dosage for optimal therapeutic level

 

Assessing theophylline toxicity

Method Name

Kinetic Interaction of Microparticles in a Solution (KIMS)

Reporting Name

Theophylline, S

Specimen Type

Serum

Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  28 days
  Ambient  72 hours

Reject Due To

Gross hemolysis Reject

Reference Values

Therapeutic:

Bronchodilation: 8.0-20.0 mcg/mL

Neonatal apnea (≤4 weeks old): 6.0-13.0 mcg/mL

Critical value: >20.0 mcg/mL

Method Description

The assay is based on the kinetic interaction of microparticles in a solution. Theophylline antibody is covalently coupled to microparticles and the drug derivative is linked to a macromolecule. The kinetic interaction of microparticles in solutions is induced by binding of drug-conjugate to the antibody on the microparticles and is inhibited by the presence of theophylline in the sample. A competitive reaction takes place between the drug conjugate and theophylline in the serum sample for binding to the theophylline antibody on the microparticles. The resulting kinetic interaction of microparticles is indirectly proportional to the amount of drug present in the sample.(Package insert: Theophylline reagent. Roche Diagnostics; 09/2017)

Day(s) Performed

Monday through Saturday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

80198

Forms

If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.