Method Description

IgM:

The anti-Borrelia burgdorferi IgM test is designed to detect IgM class antibodies to B burgdorferi in human sera. The sensitized wells of plastic microwell strips are prepared by passive adsorption with B burgdorferi whole cell antigen. Test sera are diluted with the sample diluent provided and then combined with absorbent solution. The absorbent solution contains anti-human IgG that precipitates and removes IgG and rheumatoid factor from the sample leaving IgM free to react with the immobilized antigen. During sample incubation, any antigen specific IgM antibody in the sample will bind to the immobilized antigen. The plate is washed to remove unbound antibody and other serum components. Peroxidase conjugated goat anti-human IgM (m chain specific) is added to the wells and the plate is incubated. The conjugate will react with IgM antibody immobilized on the solid phase. The wells are washed to remove unbound conjugate. The microwells containing immobilized peroxidase conjugate are incubated with peroxidase substrate solution. Hydrolysis of the substrate by peroxidase produces a color change. After a period of time the reaction is stopped, and the color intensity of the solution is measured photometrically. The color intensity of the solution depends upon the antibody concentration in the original test sample.(Package insert: Anti-B burgdorferi IgM. ZEUS Scientific; Rev Date 03/16/2026)

IgG:

The anti-B burgdorferi IgG test is designed to detect IgG class antibodies to B burgdorferi in human sera. The sensitized wells of plastic microwell strips are prepared by passive adsorption with B burgdorferi whole cell antigen. Properly diluted test sera are incubated in antigen coated microwells. Any antigen specific antibody in the sample will bind to the immobilized antigen. The plate is washed to remove unbound antibody and other serum components. Peroxidase conjugated goat anti-human IgG (Fc chain specific) is added to the wells and the plate is incubated. The conjugate will react with IgG antibody immobilized on the solid phase. The wells are washed to remove unreacted conjugate. The microwells containing immobilized peroxidase conjugate are incubated with peroxidase substrate solution. Hydrolysis of the substrate by peroxidase produces a color change. After a period of time the reaction is stopped and the color intensity of the solution is measured photometrically. The color intensity of the solution depends upon the antibody concentration in the original test sample.(Package insert: Anti-B burgdorferi IgG. ZEUS Scientific; Rev Date 03/16/2026)