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HelpOrder Code TOXCG Toxocara Antibody, IgG, Serum
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Aiding in the diagnosis of Toxocara infection
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Reporting Name
Toxocara Ab, IgG, SSpecimen Type
SerumSpecimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Frozen (preferred) | 30 days |
| Refrigerated | 5 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Heat-inactivated | Reject |
Reference Values
Negative
Reference values apply to all ages.
Method Description
Microtiter strip wells are precoated with synthetic Toxocara canis antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material, horseradish peroxidase-labeled protein A conjugate is added. This conjugate binds to the captured T canis-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding tetramethylbenzidine substrate, which gives a blue reaction product. The intensity of this product is proportional to the amount of T canis-specific antibodies in the specimen. Sulfuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450 nm is read using an enzyme-linked immunosorbent assay microwell plate reader.(Package insert: Toxocara canis ELISA IgG Test Kit. Gold Standard Diagnostics; 07/11/2023)
Day(s) Performed
Tuesday, Thursday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86682