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Order Code TPO Thyroperoxidase Antibodies, Serum

Additional Codes

Mayo Test ID
TPO

Reporting Name

Thyroperoxidase Ab, S

Useful For

Aiding in the diagnosis of thyroid autoimmune disorders

 

Differentiating thyroid autoimmune disorders from nonautoimmune goiter or hypothyroidism

 

As a diagnostic tool in deciding whether to treat a patient who has subclinical hypothyroidism

Specimen Type

Serum Red


Specimen Required


Patient Preparation: For 12 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.

Supplies: Sarstedt 5 mL Aliquot Tube (T914)

Collection Container/Tube: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.6 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial within 2 hours of collection.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 7 days
  Frozen  30 days
  Ambient  7 days

Reject Due To

Gross hemolysis Reject
Gross lipemia OK

Reference Values

<9.0 IU/mL

Reference values apply to all ages.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Day(s) Performed

Monday through Saturday

Method Name

Chemiluminometric Immunoassay

CPT Code Information

86376

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TPO Thyroperoxidase Ab, S 8099-4

 

Result ID Test Result Name Result LOINC Value
TPO Thyroperoxidase Ab, S 8099-4

Method Description

The Access thyroperoxidase (TPO) antibody assay is a sequential 2-step immunoenzymatic (sandwich) assay. A sample is added to a reaction vessel with paramagnetic particles coated with thyroperoxidase protein. The serum TPO antibody binds to the thyroperoxidase. After incubation in a reaction vessel, materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Protein A-alkaline phosphatase conjugate is added and binds to the TPO antibody. After the second incubation, materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. The chemiluminescent substrate Lumi-Phos 530 is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is directly proportional to the concentration of TPO antibody in the sample. The amount of analyte in the sample is determined from a stored, multipoint calibration curve. The analyte in the calibrator is traceable to international standard WHO 66/387.(Package insert: TPO antibody. Beckman Coulter, Inc; 06/2020)

Testing Algorithm

For more information, see Thyroid Function Ordering Algorithm