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HelpOrder Code TRYPT Tryptase, Serum
Additional Codes
| Mayo Test ID |
|---|
| TRYPT |
Reporting Name
Tryptase, SUseful For
Evaluation of individuals with suspected mast cell activation, which may occur as a result of anaphylaxis or allergen challenge
Evaluation of patients with suspected mast cell activation syndrome
Evaluation of patients with suspected cutaneous or systemic mastocytosis
Specimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial
Additional Information: Tryptase degenerates very quickly when left in the presence of red blood cells.
Specimen Minimum Volume
0.2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Frozen (preferred) | 14 days |
| Refrigerated | 7 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
<11.5 ng/mL
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Day(s) Performed
Monday through Friday
Method Name
Fluorescence Enzyme Immunoassay (FEIA)
CPT Code Information
83520
Method Description
Anti-tryptase, covalently coupled to ImmunoCAP, reacts with tryptase in the patient serum specimen. After washing, enzyme-labeled antibodies against tryptase are added to form a complex. After incubation, unbound enzyme-labeled antibodies are washed away, and the bound complex is incubated with a developing agent. After stopping the reaction, the fluorescence in the eluate is measured. The fluorescence is directly proportional to the concentration of tryptase in the serum specimen.(Package insert: ImmunoCAP Tryptase. Phadia AB; 10/2019)
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.
Testing Algorithm
For more information see Mast Cell Disorder: Diagnostic Algorithm, Bone Marrow.