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HelpOrder Code UHBAG Histoplasma and Blastomyces Antigen, Enzyme Immunoassay, Urine
Specimen Required
Supplies: Sarstedt Aliquot tube, 5 mL (T914)
Container/Tube: Plastic vial
Specimen Volume: 3 mL
Collection Instructions:
1. Collect a random urine specimen, with no preservative.
2. Do not centrifuge to remove particulates.
Useful For
Diagnosing Histoplasma capsulatum or Blastomyces dermatitidis infection, without differentiation between the organisms, using random urine specimens
Monitor antigen levels following initiation of antifungal treatment
Method Name
Enzyme Immunoassay (EIA)
Reporting Name
Histoplasma/Blastomyces Ag, EIA, USpecimen Type
UrineSpecimen Minimum Volume
2.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Urine | Refrigerated (preferred) | 14 days |
| Frozen | 28 days | |
| Ambient | 7 days |
Reject Due To
| Gross hemolysis | Reject |
| Turbid Colored |
Reject |
Reference Values
Histoplasma/Blastomyces Antigen Result:
Not detected
Histoplasma/Blastomyces Antigen Value:
Not detected: 0.0 ng/mL
Detected: <1.3 ng/mL
Detected: 1.3-20.0 ng/mL
Detected: >20.0 ng/mL
Reference values apply to all ages.
Method Description
The assay detects Blastomyces dermatitidis antigen in human serum samples using specific, proprietary antibodies in an enzyme-linked immunosorbent assay format. The detection method involves an enzyme/substrate system with the level of urinary B dermatitidis antigen proportional to the assay signal. The patient specimen result is compared to a cutoff calibrator and a standard curve of a series of assay calibrators (1.25 to 20.00 ng/mL) to determine the presence or absence of antigen and, if present, to establish a quantitative level of B dermatitidis serum antigen.(Package insert: Blastomyces dermatitidis Urinary Antigen Detection Kit. Gotham Biotechnology; V1, R3, 06/2021)
Day(s) Performed
Monday through Sunday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87449
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.