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Order Code ULFA Cryptococcus Antigen Screen, Lateral Flow Assay, Random, Urine


Specimen Required


Supplies: Urine Tubes, 10 mL (T068)

Submission Container/Tube: Plastic, 10-mL urine tube

Specimen Volume: 1 mL

Collection Instructions:

1. Collect a random urine specimen.

2. No preservative.


Useful For

Aiding in the diagnosis of infection with Cryptococcus neoformans or Cryptococcus gattii

 

This test should not be used as a test of cure.

 

This test should not be used as a screening procedure for the general population.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
ULFAT Cryptococcus Ag Titer, LFA, U No No

Testing Algorithm

If this screen is positive, the antigen titer will be performed at an additional charge.

Method Name

Lateral Flow Assay (LFA)

Reporting Name

Cryptococcus Ag Screen, LFA, U

Specimen Type

Urine

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Urine Refrigerated (preferred) 14 days
  Frozen  14 days

Reject Due To

Gross hemolysis Reject
Turbid; Colored Reject

Reference Values

Negative

Reference values apply to all ages.

Method Description

The Cryptococcus antigen (CrAg) lateral flow assay is a sandwich immunochromatographic assay. Specimens and diluent are added to a test tube, and the lateral flow device is added. The test uses specimen wicking to capture gold-conjugated anti-cryptococcal antigen monoclonal antibodies and gold-conjugated control antibodies deposited on the test membrane. If cryptococcal antigen is present in the specimen, it binds to the gold-conjugated anti-cryptococcal antigen antibodies. This complex wicks up the membrane and interacts with the test line, which has immobilized anti-cryptococcal antigen monoclonal antibodies. The antigen-antibody complex forms a sandwich at the test line causing a visible line to form. A valid test shows a visible line at the control line. Positive test results create 2 lines (control and specimen), while negative results form only the control line.(Package insert: CrAg Lateral Flow Assay. IMMY; Rev 06/27/2019)

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87899

87899 (if appropriate)

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.