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Order Code VITAE Vitamin A and Vitamin E, Serum


Shipping Instructions


Ship specimen in amber vial to protect from light.



Specimen Required


Patient Preparation: Patient should fast overnight (12-14 hours); infants should have specimen collected before next feeding.

Supplies: Amber Frosted Tube, 5 mL (T915)

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Amber vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into light protected plastic vial within 2 hours of collection.


Useful For

Diagnosing vitamin A deficiency and toxicity

 

Evaluating persons with intestinal malabsorption of lipids

 

Monitoring of Vitamin E supplementation/treatment

Profile Information

Test ID Reporting Name Available Separately Always Performed
VITAP Vitamin A, S Yes, (Order VITA) Yes
VITE Vitamin E, S Yes Yes

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Vitamin A and Vitamin E, S

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 28 days LIGHT PROTECTED
  Frozen  28 days LIGHT PROTECTED
  Ambient  7 days LIGHT PROTECTED

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK

Reference Values

VITAMIN A (RETINOL)

0-6 years: 11.3-64.7 mcg/dL

7-12 years: 12.8-81.2 mcg/dL

13-17 years: 14.4-97.7 mcg/dL

≥18 years: 32.5-78.0 mcg/dL

 

VITAMIN E (ALPHA-TOCOPHEROL)

0-17 years: 3.8-18.4 mg/L

≥18 years: 5.5-17.0 mg/L

Day(s) Performed

Sunday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

84446

84590

Method Description

Deuterated vitamin A (d6-all-trans retinol) is added to serum as an internal standard. Vitamin A (all-trans retinol) and the deuterated internal standard are extracted from the specimens using on-line turbulent flow high performance liquid chromatography and analyzed by liquid chromatography tandem mass spectrometry using multiple reaction monitoring in positive mode.(Unpublished Mayo method)

 

Deuterated vitamin E (d6-alpha-tocopherol) is added to serum as an internal standard. Vitamin E (alpha-tocopherol) and the deuterated internal standard are extracted from the specimens and analyzed by liquid chromatography tandem mass spectrometry.(Unpublished Mayo method)