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HelpOrder Code VITK1 Vitamin K1, Serum
Useful For
Assessing circulating vitamin K1 concentration
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Vitamin K1, SSpecimen Type
SerumSpecimen Required
Patient Preparation: Fasting overnight (12-14 hours) (infants-collect prior to next feeding).
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 2 mL
Collection Instructions: Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.75 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 30 days | |
| Ambient | 30 days | ||
| Frozen | 30 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | Reject |
| Gross icterus | OK |
Reference Values
<18 years: Not established
≥18 years: 0.10-2.20 ng/mL
Method Description
Deuterated stable isotope (vitamin K1-d7) is added to a serum sample as an internal standard. Protein is precipitated from the mixture by the addition of ethanol. Vitamin K1 and internal standard are extracted from the resulting supernatant by solid-phase extraction. Vitamin K1 and internal standard are then separated utilizing high-throughput liquid chromatography with analysis on a tandem mass spectrometer equipped with a heated nebulizer ion source using multiple-reaction monitoring.(Unpublished Mayo method)
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
84597
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.