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Order Code VRERP Vancomycin-Resistant Enterococcus, Molecular Detection, PCR, Varies

Reporting Name

VRE PCR

Useful For

Identifying carriers of vancomycin-resistant enterococci

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by vancomycin-resistant Enterococcus DNA is unlikely.

 

Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Perianal, perirectal, rectal, anal

Supplies: Culturette (BBL Culture Swab) (T092)

Container/Tube: Culture transport swab (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)

Specimen Volume: Swab

Specimen Stability Information: Refrigerated (preferred)/Frozen

 

Acceptable:

Specimen Type: Preserved feces

Supplies: Culture and Sensitivity Stool Transport Vial (T058)

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair, Para-Pak C and S vial)

Specimen Volume: Representative portion of feces

Collection Instructions:

1. Collect fresh feces and submit 1 gram or 5 mL in container with transport medium.

2. Place feces in preservative within 2 hours of collection.

Specimen Stability Information: Ambient (preferred)/ Refrigerated

 

Specimen Type: Unpreserved feces

Supplies:

-Stool Container, Small (Random), 4 oz Random (T288)

-Stool Collection Kit, Random (T635)

Container/Tube: Fecal container

Specimen Volume: Representative portion of feces

Collection Instructions: Collect fresh fecal specimen and submit representative sample in fecal container.

Specimen Stability Information: Refrigerated (preferred)/Frozen


Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Varies

Reference Values

Negative

Day(s) Performed

Monday through Sunday

CPT Code Information

87500

Method Description

Bacterial DNA is extracted from the specimen. Using the LightCycler instrument and fluorescent energy transfer probes, vanA and vanB genes responsible for vancomycin resistance in enterococci are detected directly from perianal swabs or fecal specimens.(Sloan LM, Uhl JR, Vetter EA, et al: Comparison of the Roche LightCycler vanA/vanB detection assay and culture for detection of vancomycin-resistant enterococci from perianal swabs. J Clin Microbiol 2004 Jun;42(6):2636-2643. doi: 10.1128/JCM.42.6.2636-2643.2004; Hefazi M, Damlaj M, Alkhateeb HB, et al: Vancomycin-resistant Enterococcus colonization and bloodstream infection: prevalence, risk factors, and the impact on early outcomes after allogeneic hematopoietic cell transplantation in patients with acute myeloid leukemia. Transplant Infect Dis. 2016 Dec;18(6):913-920. doi: 10.1111/tid.12612)

Reject Due To

E-swab, calcium alginate or, cotton-tipped swab
Swab sent in gel transport medium, viral or universal transport medium
Dry swab
Formalin or PVA fixative
Reject

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Specimen Minimum Volume

See Specimen Required