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Order Code WNGC West Nile Virus Antibody, IgG, Spinal Fluid

Method Name

Only orderable as part of a profile. For more information see WNC / West Nile Virus Antibody, IgG and IgM, Spinal Fluid.

 

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

West Nile Virus Ab, IgG, CSF

Specimen Type

CSF


Specimen Required


Only orderable as part of a profile. For more information see WNC / West Nile Virus Antibody, IgG and IgM, Spinal Fluid.

 

Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Collection Container/Tube: Sterile vial

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL


Specimen Stability Information

Specimen Type Temperature Time Special Container
CSF Refrigerated (preferred) 7 days
  Frozen  30 days

Reference Values

Only orderable as part of a profile. For more information see WNC / West Nile Virus Antibody, IgG and IgM, Spinal Fluid.

 

IgG: Negative

Reference values apply to all ages.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86789

Specimen Minimum Volume

0.8 mL

Useful For

Aids in diagnosing recent or past central nervous system West Nile virus infection

Reject Due To

Gross hemolysis Reject

Method Description

Polystyrene microwells are coated with recombinant West Nile virus antigen. Diluted serum specimens and controls are incubated in the wells to allow specific antibody present in the specimens to react with the antigen. Nonspecific reactants are removed by washing, and peroxidase-conjugated antihuman IgG is added and reacts with specific IgG. Excess conjugate is removed by washing. Enzyme substrate and chromogen are added, and the color is allowed to develop. After adding the Stop reagent, the resultant color change is quantified by a spectrophotometric reading of optical density (OD). Specimen OD readings are compared with reference cutoff readings to determine results.(Package insert: West Nile Virus IgG DxSelect. Focus Diagnostics; 05/08/2018)

Day(s) Performed

Monday, Wednesday, Friday