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HelpOrder Code reflex only MPLR MPL Exon 10 Mutation Detection, Reflex, Varies
Additional Codes
MAYO CODE MPLR
Reporting Name
MPL Exon 10 Mutation Detection, RSpecimen Type
VariesSpecimen Required
Only orderable as a reflex. For more information see MPNR / Myeloproliferative Neoplasm, JAK2 V617F with Reflex to CALR and MPL, Varies.
Submit only 1 of the following specimens:
Specimen Type: Whole Blood
Container/Tube: Lavender top (EDTA) or yellow top (ACD solution B)
Specimen Volume: 3 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot.
3. Label specimen as whole blood.
Specimen Stability Information: Ambient (preferred)/Refrigerate
Specimen Type: Bone marrow
Container/Tube: Lavender top (EDTA) or yellow top (ACD solution B)
Specimen Volume: 2 mL
Collection Instructions:
1. Invert several times to mix bone marrow.
2. Send bone marrow specimen in original tube. Do not aliquot.
3. Label specimen as bone marrow.
Specimen Stability Information: Ambient (preferred)/Refrigerate
Specimen Type: Extracted DNA from whole blood or bone marrow
Container/Tube: 1.5- to 2-mL tube
Specimen Volume: Entire specimen
Collection Instructions:
1. DNA must be extracted from blood or bone marrow within 7 days of collection.
2. Label specimen as extracted DNA and source of specimen
3. Provide volume and concentration of the DNA.
Specimen Stability Information: Frozen (preferred) 1 year/Refrigerate/Ambient
Additional Information: DNA must be extracted in a CLIA-certified laboratory or equivalent and must be extracted from a specimen type listed as acceptable for this test (including applicable anticoagulants). We cannot guarantee that all extraction methods are compatible with this test. If testing fails, one repeat will be attempted, and if unsuccessful, the test will be reported as failed and a charge will be applied.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Varies | 7 days |
Reference Values
Only orderable as a reflex. For more information see MPNR / Myeloproliferative Neoplasm, JAK2 V617F with Reflex to CALR and MPL, Varies.
An interpretive report will be provided.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
81339-MPL (myeloproliferative leukemia virus oncogene, thrombopoietin receptor, TPOR) (eg, myeloproliferative disorder), exon 10 sequence
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MPLR | MPL Exon 10 Mutation Detection, R | 62948-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 36672 | Final Diagnosis | 22637-3 |
Method Name
Only orderable as a reflex. For more information see MPNR / Myeloproliferative Neoplasm, JAK2 V617F with Reflex to CALR and MPL, Varies.
Sanger Sequencing
Useful For
Aiding in the distinction between a reactive cytosis and a chronic myeloproliferative disorder
Evaluates for mutations in MPL in an algorithmic process for the MPNR / Myeloproliferative Neoplasm, JAK2 V617F with Reflex to CALR and MPL, Varies
Reject Due To
| Gross hemolysis | Reject |
| Paraffin embedded bone marrow aspirate clot or biopsy blocks | Reject |
| Slides | Reject |
| Paraffin shavings | Reject |
| Moderately to severely clotted | Reject |
Method Description
Genomic DNA is extracted from bone marrow, and MPL exon 10 amplified using standard polymerase chain reaction. The entire exon 10 sequence is obtained using Sanger sequencing (BigDye terminator V1.1 cycle sequencing kit from Applied Bioscience) with analysis on an automated genetic analyzer.(Unpublished Mayo method)
Day(s) Performed
Monday through Friday